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FOXO1/FKHR antibody (pAb)
| RRID: AB_2614952 |
| Aliases: FKH1, FOXO1A |
| Catalog No: 39629 | Format: 200 µl | ¥86,000 | Buy |
| Catalog No: 39630 | Format: 10 µl | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution
Immunogen
This FOXO1/FKHR antibody was raised against a peptide from the C-terminal region of human FOXO1/FKHR.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
FOXO1/FKHR antibody (pAb) tested by Immunoprecipitation.
10 µl of FOXO1/FKHR antibody was used to immunoprecipitate FOXO1/FKHR from 500 µg of K562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FOXO1/FKHR antibody at a dilution of 1:500.
FOXO1/FKHR antibody (pAb) tested by Western blot. U2OS nuclear extract (20 µg per lane) was probed with 39629 at a 1:500 dilution.
Background
FOXO1/FKHR (forkhead box 01) belongs to the forkhead family of transcription factors that contain a distinct forkhead domain. Chromosomal aberrations involving FOXO1, such as translocation with PAX3, are associated with aveolar rhabdomyosarcoma. The specific function of this gene is undetermined.
Positive Control
Nuclear extracts from U2OS cells can be used as a positive control for FOXO1/FKHR antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
