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Histone H3T11ph antibody (pAb)
| RRID: AB_2793549 |
| 商品コード: 61197 | Format: 100 µg | ¥66,800 | Buy |
| 商品コード: 61198 | Format: 10 µg | ¥18,800 | Buy |
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Applications
Immunogen
This Histone H3 phospho Thr11 antibody was raised against a peptide containing phospho-Thr11 of human histone H3.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39151) of this antibody is also available.
View our Guide to Histone Modifications and Biological Function.
Histone H3 phospho Thr11 pAb tested by Western blot.
Western blot probed with Histone H3 phospho Thr11 pAb (1 µg/ml dilution).
Lane 1: 20 µg nuclear extract of HeLa cells.
Lane 2: 20 µg nuclear extract of HeLa cells treated with colcemid.
Histone H3 phospho Thr11 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr11 pAb for phospho-Thr11 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 0.5 µg/ml.
Lane 1: Peptide phosphorylated at threonine 11. Lane 2: Unmodified threonine 11 peptide. Lane 3: Peptide phosphorylated at threonine 3. Lane 4: Peptide phosphorylated at threonine 6. Lane 5: Peptide phosphorylated at threonine 45. Lane 6: Peptide phosphorylated at threonine 80. Lane 7:Peptide phosphorylated at threonine 10. Lane 8:Peptide phosphorylated at threonine 28.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
