Histone H3S10ph antibody (pAb)

RRID: AB_2793206
Catalog No: 39253 Format: 100 µl ¥88,000 Buy
Catalog No: 39254 Format: 10 µl ¥24,000 Buy

Request a quote for a bulk order Request Quote


Product Review Submit a Review


Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 10 µl per ChIP
ICC/IF: 1:500 - 1:2,000 dilution
WB: 1:5,000 - 1:50,000 dilution

For Histone H3S10ph, we also offer AbFlex® Histone H3S10ph Recombinant Antibody (rAb). For details, see Catalog No. 91131.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB
IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H3 phospho Ser10 antibody was raised against a peptide including phospho-serine 10 of histone H3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 

Histone H3 phospho Ser10 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H3 phospho Ser10 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the GAPDH gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H3 phospho Ser10 antibody tested by immunofluorescence.
Staining of HeLa cells with Histone H3 phospho Ser10 antibody (1:2,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

Histone H3 phospho Ser10 antibody tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H3 phospho Ser10 antibody at a dilution of 1:20,000.
    Lane 1: No treatment.
    Lane 2: Cells treated with colcemid to arrest cells at mitosis.

Histone H3 phospho Ser10 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 antibody for histone H3 phospho Ser10. Phosphorylated peptides corresponding to the immunogen and a related sequence derived from histone H3 were spotted onto PVDF and probed with the antibody at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: phospho-Ser10 peptide.
    Lane 2: unmodified Ser10 peptide.
    Lane 3: phospho-Ser28 peptide 4 peptide.
    Lane 4: unmodified Ser28 peptide.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is strongly phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.

Positive Control

Active Motif's HeLa acid extract (PTX treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Ser10 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3S10ph antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting