Histone H3K14me1 antibody (pAb)

RRID: AB_2793664
商品コード: 61509 Format: 100 µl ¥66,800 Buy
商品コード: 61510 Format: 10 µl ¥18,800 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated

Immunogen

This antibody was raised against a peptide including monomethyl-lysine 14 of human histone H3.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H3K14me1 antibody (pAb) tested by Western blot.

Histone H3K14me1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg) probed with H3K14me1 antibody at a 1:500 dilution.

Histone H3K14me1 antibody (pAb) tested by dot blot analysis.

Specificity Data:
Dot blot analysis was used to confirm the specificity of Histone H3K14me1 antibody (pAb) for monomethyl-lysine 14 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1:30,000. The amount of peptide (in picomoles) spotted is indicated next to each row.Top panel - Lane 1: unmodified Lys14. Lane 2: monomethyl Lys14. Lane 3: dimethyl Lys14. Lane 4: trimethyl Lys14. Middle panel - Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: unmodified Lys79. Lane 10: monomethyl Lys79. Lane 11: dimethyl Lys79. Lane 12: trimethyl Lys79. Bottom panel - Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36.

Histone H3K14me1 antibody (pAb) tested by immunofluorescence.

Detection of H3K4me1 by immunofluorescence
U2OS cells were stained with H3K4me1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H3K4me1 antibody staining. Right panel: merge.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting