AbFlex® Histone H3K14ac antibody (rAb)

RRID: AB_3216370
商品コード: 91397 Format: 100 µg ¥72,000 Buy
商品コード: 91398 Format: 10 µg ¥20,000 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
17 kDa
Reactivity:
Mouse, Wide Range Predicted

Applications

Western Blot Validated Enzyme-linked Immunosorbent Assay Validated

Immunogen

This Histone H3 acetyl Lys14 antibody was raised against a peptide including acetyl-lysine 14 of histone H3.

Buffer

Purified antibody is in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.

 

AbFlex® Histone H3K14ac antibody (rAb) tested by Luminex bead-based specificity analysis.
Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex® Histone H3K14ac antibody (rAb) antibody. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlex® Histone H3K14ac antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument

Luminex® is a registered trademark of Luminex Corporation.

AbFlex® Histone H3 acetyl Lys14 (rAb) antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with Histone H3 acetyl Lys14 antibody at 2 micro;g/ml.
    Lane 1: Sodium Butyrate
    Lane 2: No treatment.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting