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Recombinant HDAC1 protein
| Aliases: HD1, RPD3, GON-10, RPD3L1 |
| 商品コード: 31504 | Format: 50 µg | ¥93,100 | Buy |
| 商品コード: 31908 | Format: 1 mg | ¥788,500 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Protein Details
Recombinant human HDAC1 was expressed in Sf9 cells as the full length protein (accession number NP_004955.2) with a C-terminal FLAG-Tag. The molecular weight of the protein is 56 kDa. The recombinant protein is >65% pure by SDS-PAGE.
References
This product was used in the following publications:
Bio. Protoc. (2018). 8(14): pii: e2924. PMID: 30283810.
Nat Commun. (2018). Jan 9;9(1):105. PMID: 29317660.
Recombinant HDAC1 protein gel.
HDAC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
MW: 56 kDa
Purity> >65%
Recombinant HDAC1 activity by ELISA Assay.
1 μM H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 20 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature. Reaction product was detected with anti-H3K9me0 antibody (Cat #: 91155) via ELISA.
Recombinant HDAC1 activity by ELISA Assay.
1 μM H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 20 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature. Reaction product was detected with anti-H3K9me0 antibody (Cat #: 91155) via ELISA.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.This product is for research use only and is not for use in diagnostic procedures.
