Recombinant HDAC2 protein

Aliases: HD2, RPD3, YAF1
商品コード: 31505 Format: 50 µg ¥96,000 Buy
商品コード: 31909 Format: 1 mg ¥788,500 Buy

Request a quote for a bulk order Request Quote


Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Protein Details

Recombinant human HDAC2 was expressed in Sf9 cells as the full length protein (accession number NP_001518.3) with a C-terminal FLAG-Tag. The molecular weight of the protein is 60 kDa. The recombinant protein is >80% pure by SDS-PAGE.

References


This product was used in the following publications:
Cell Rep. (2018). 24(13): 3488-3502. PMID: 30257210. Histone Deacetylase (HDAC) Assay.

 

Recombinant HDAC2 protein gel.
HDAC2 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.
MW: 60 kDa
Purity: > 45%

Recombinant HDAC2 activity assay.
1 µM H3K9ac peptide was incubated with different concentrations of HDAC2 protein in a reaction system for 30 min at 37°C, then H3K9me0 antibody and SA-XL665 mixture was added for 1 h at room temperature. HTRF assay was used for detection. 

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.