Recombinant HDAC6 (597-728, deleted mutant) protein

Aliases: HD6, JM21, CPBHM, PPP1R90
商品コード: 31506 Format: 20 µg ¥90,000 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Protein Details

Recombinant HDAC6 (597-728, deleted mutant) deletes the second catalytic domain. This recombinant protein was expressed in a baculovirus expression system (accession number NP_006035.2) with an N-terminal 6xHis Tag and FLAG-Tag. The molecular weight of the protein is 122.1 kDa. The recombinant protein is >80% pure by SDS-PAGE.

 

Recombinant HDAC6 (597-728, deleted mutant) protein gel.
HDAC6 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

Recombinant HDAC6 (597-728, deleted mutant) protein activity assay.
3 μM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (597-728, deleted mutant) in reaction buffer for 30 minutes at 37°C followed by developing for 30 minutes at room temperature.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.