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Recombinant HDAC8 protein
| Aliases: Histone Deacetylase 8, HDACL1, HD8, CDA07, CDLS5, MRXS6, RPD3 |
| 商品コード: 31536 | Format: 20 µg | ¥90,000 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Protein Details
Recombinant human HDAC8 was expressed in a baculovirus expression system as the full length protein (accession number NP_060956.1) with a C-terminal FLAG-Tag. The molecular weight of the protein is 43.8 kDa. The purity of HDAC8 protein is >90% by SDS-PAGE.
Recombinant HDAC8 protein gel.HDAC8 protein was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.
MW: 43.8 kDa
Purity: >92%
HTRF assay for HDAC8 protein activity.
1 µM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein in reaction buffer for 30 min at 37°C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.
HTRF assay for HDAC8 protein activity.
1 µM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein in reaction buffer for 30 min at 37°C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
