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Recombinant HDAC8 protein, His-Tag
| Aliases: Histone Deacetylase 8, HDACL1, HD8, CDA07, CDLS5, MRXS6, RPD3 |
| 商品コード: 31566 | Format: 50 µg | ¥90,000 | Buy |
| 商品コード: 31966 | Format: 1 mg | ¥788,500 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Protein Details
Recombinant human HDAC8 protein, His-Tag was expressed in a baculovirus expression system as the full length protein (accession number NP_060956.1) with a C-terminal 6xHis Tag. The molecular weight of the protein is 43.8 kDa. The HDAC8 protein, His-Tag is >90% pure by SDS-PAGE.
Recombinant HDAC8 His-Tag protein gel. HDAC8 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
HTRF assay for HDAC8 protein, His-Tag
3 µM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein, His-Tag in reaction buffer for 30 min at 37°C, Reaction product was detected by Anti-H3K9me0-Eu antibody.
HTRF assay was used for activity detection.
HTRF assay for HDAC8 protein, His-Tag
3 µM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein, His-Tag in reaction buffer for 30 min at 37°C, Reaction product was detected by Anti-H3K9me0-Eu antibody.
HTRF assay was used for activity detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
