Recombinant Histone TH2B
|Aliases: Testis-specific H2B, Th2B, Histone variant|
|Catalog No: 31577||Format: 100 µg||¥32,000||Buy|
|Catalog No: 31977||Format: 1 mg||¥152,000||Buy|
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Expressed In: E. coli Protein Species: Human
A representative Technical Data Sheet (TDS) is provided here. Please refer to your lot-specific TDS upon arrival for specific buffer contents and lot-specific protein concentration.
TH2B (testis-specific H2B) is originally identified as a testis-specific variant of histone H2B. TH2A is a testis-specific variant of histone H2A. They are abundant in the testis, oocytes and fertilized eggs. TH2A and TH2B are controlled by a shared promoter that is located between them. Disruption of Th2a and Th2b genes causes defects in spermatogenesis. TH2A/TH2B may enhance reprogramming by introducing processes that normally operate in zygotes and during SCNT. TH2A and TH2B induce nucleosome instability. TH2B controls the chromatin-to-nucleoprotamine transition.
Recombinant histone TH2B is suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.
Recombinant Histone TH2B (accession number NP_733759.1) was expressed in E. coli cells as full length protein without any tag. The molecular weight of TH2B is about 14.2 kDa. The recombinant histone is >95% pure by SDS-PAGE.
Protein Sequence: PEVSSKGATI SKKGFKKAVV KTQKKEGKKR KRTRKESYSI YIYKVLKQVH PDTGISSKAM SIMNSFVTDI FERIASEASR LAHYSKRSTI SSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSSK
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.