Recombinant Histone TH2B

Aliases: Testis-specific H2B, Th2B, Histone variant
Catalog No: 31577 Format: 100 µg ¥32,000 Buy
Catalog No: 31977 Format: 1 mg ¥152,000 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to your lot-specific TDS upon arrival for specific buffer contents and lot-specific protein concentration.

Background

TH2B (testis-specific H2B) is originally identified as a testis-specific variant of histone H2B. TH2A is a testis-specific variant of histone H2A. They are abundant in the testis, oocytes and fertilized eggs. TH2A and TH2B are controlled by a shared promoter that is located between them. Disruption of Th2a and Th2b genes causes defects in spermatogenesis. TH2A/TH2B may enhance reprogramming by introducing processes that normally operate in zygotes and during SCNT. TH2A and TH2B induce nucleosome instability. TH2B controls the chromatin-to-nucleoprotamine transition.

Application Notes

Recombinant histone TH2B is suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.

Protein Details

Recombinant Histone TH2B (accession number NP_733759.1) was expressed in E. coli cells as full length protein without any tag. The molecular weight of TH2B is about 14.2 kDa. The recombinant histone is >95% pure by SDS-PAGE.

Protein Sequence: PEVSSKGATI SKKGFKKAVV KTQKKEGKKR KRTRKESYSI YIYKVLKQVH PDTGISSKAM SIMNSFVTDI FERIASEASR LAHYSKRSTI SSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSSK

 

Recombinant Histone TH2B gel.
Histone TH2B was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.