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Recombinant Histone H3K9me3 (MLA)
| 商品コード: 31601 | Format: 100 µg | ¥63,000 | Buy |
| 商品コード: 31701 | Format: 1 mg | ¥189,000 | Buy |
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Expressed In: E. coli Protein Species: Human
Methylated Lysine Analog
Recombinant Histone H3K9me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ϒ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
Recombinant Histone H3K9me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ϒ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant human Histone H3 trimethyl Lys9 (H3K9me3) (accession number NP_003520.1) was expressed in E. coli cells and contains two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110). Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). The molecular weight of the protein is 15285 Daltons.
References
Human
Recombinant Histone H3K9me3 (MLA) protein gel.
12.5% SDS-PAGE Coomassie staining.
Purity: > 95%
Western Blot analysis for Recombinant Histone H3K9me3 (MLA)
Unmodified Histone H3.1 (Lane 1) and Recombinant Histone H3K9me3 (MLA) (Lane 2) were detected with anti-H3K9me3 antibody. Only Recombinant Histone H3K9me3 (MLA) can be detected by anti-H3K9me3 antibody.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
