HeLa acid extract

Catalog No: 36200 Format: 100 µg ¥32,000 Buy

Contents

100 µg HeLa acid extract at 0.5 µg/µl.

Background

HeLa acid extract was prepared under acidic conditions from cells harvested from a cell culture of the HeLa human epithelial carcinoma cell line. Acid extraction is performed under conditions that are optimized for solubility of histone proteins.

The HeLa cell line was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells.

Application Notes

Acid extracts are commonly utilized for isolation and purification of histones. HeLa acid extract is recommended for use in various applications across many research areas, including cancer, virology, and gene mapping studies.

Extract Origin

Human Cervix Adenocarcinoma

Extract Composition

HeLa acid extracts are supplied in dilution buffer (PBS supplemented with 0.5 mM DTT and 2 mM PMSF).

Quality Control

Acid extracts have been quality control tested by Western blot.

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.