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Histone H4ac (pan-acetyl) antibody (pAb)
| RRID: AB_2793201 |
| 商品コード: 39243 | Format: 100 µl | ¥92,000 | Buy |
| 商品コード: 39244 | Format: 10 µl | ¥25,000 | Buy |
| 商品コード: 39043 | Format: 50 µl | ¥55,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-Seq, ChIP-qPCR
WB
ICC/IF
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena histone H2A acetylated at multiple lysines. (The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts).
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39925) of this antibody that was purified by Protein A Chromatography is also available.
View our Guide to Histone Modifications and Biological Function.
Histone H4 pan-acetyl antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 2 µl of Histone H4 pan-acetyl pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H4 pan-acetyl antibody (pAb) tested by immunofluorescence.
Staining of HeLa cells with Histone H4 pan-acetyl pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H4 pan-acetyl antibody (pAb) tested by Western blot.
HeLa nuclear extract (20 µg /lane) probed with Histone H4 pan-acetyl polyclonal antibody (1:20,000 dilution).
Lane 1: Cells treated with sodium butyrate
Lane 2: Untreated cells.
Histone H4 pan-acetyl antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl pAb for acetyl histone H4. Decreasing amounts of acetylated peptides corresponding to the immunogen, a related sequence, as well as unacetylated histone H4 peptides were spotted onto PVDF and probed with the antibody at a 1:10,000 dilution.
Lane 1: AGG[acK]GG[acK]GMG[acK]VGA[acK]RHSC
Lane 2: AGG[acK]GG[acK]GG[acK]GG[acK]GG[acK]GGC
Lane 3: SGRGKGGKGLC
Lane 4: GKGGAKRHRKC
qPCR Primers
Active Motif's Human Positive Control Primer Set ACTB-1 (Cat. No. 71003) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-1 (Cat. No. 71015) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4ac (pan-acetyl) antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
