alpha Tubulin antibody (mAb)

RRID: AB_2793243
Clone: 5-B-1-2
Catalog No: 39527 Format: 100 µl ¥70,000 Buy
Catalog No: 39528 Format: 10 µl ¥20,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Ascites
Host:
Mouse
Molecular Weight:
52 kDa
Reactivity:
Human, Mouse, Wide Range Predicted

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,500 dilution
ICC/IF: 1:1,000 - 1:10,000 dilution



Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This alpha Tubulin antibody was raised against total protein purified from sea urchin sperm axonemes.

Buffer

Ascites containing 30% glycerol and 0.035% sodium azide.

References

 

alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence.
Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain™ Immunofluorescence Tools.

alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot.
HeLa whole-cell extract (15 µg/lane) probed with alpha Tubulin mAb (1:5,000 dilution).

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of α-tubulin and β-tubulin. Alpha Tubulin expression is reduced in cells deficient in MeCP2, leading to conditions such as Rett syndrome and autistic-spectrum disorders.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for alpha Tubulin antibody (Clone 5-B-1-2).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting