SMRT / NCoR2 antibody (pAb)

RRID: AB_2793365
Aliases: TRAC, CTG26, SMRTE, TRAC1, N-CoR2, TNRC14, TRAC-1, SMAP270, SMRTE-tau
Catalog No: 39845 Format: 100 µl ¥86,000 Buy
Catalog No: 39846 Format: 10 µl ¥24,000 Buy

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
460 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution

Immunogen

This SMRT / NCoR2 antibody was raised against a peptide within the N-terminal region of SMRT / NCoR2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
SMRT / NCoR2 antibody (pAb) tested by Western blot.

SMRT / NCoR2 antibody (pAb) tested by Western blot.
Whole cell extract of HeLa cells (40 µg per lane) probed with SMRT / NCoR2 antibody (pAb) (1:500 dilution).

Background

SMRT / NCoR2 (Silencing Mediator of Retinoic acid and Thyroid hormone receptor, Nuclear receptor CoRepressor 2, SMAP270, CTG26) is a co-repressor of transcription that associates with a subset of nuclear receptors including RAR, PR, GR and TR in the absence of ligand. It is part of a large co-repressor protein complex that includes SIN3A/B and histone deacetylases HDAC1 and HDAC2.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for SMRT / NCoR2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting