Ago2 antibody (mAb)

RRID: AB_2793369
Clone: 1B1-E2H5
Aliases: Q10, EIF2C2, PPD
商品コード: 39853 Format: 100 µg ¥66,800 Buy
商品コード: 39854 Format: 10 µg ¥18,800 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
100 kDa
Reactivity:
Human

Applications

Immunoprecipitation Validated Immunohistochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IP
RNA Immunoprecipitation (RIP)
IHC(P)

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Ago2 antibody was raised against a recombinant protein corresponding to amino acids 1-148 of human Ago2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

Argonaute 2 (Ago2, aka EIF2C2) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting