BLIMP1 / PRDM1 antibody (pAb)

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This BLIMP1 / PRDM1 antibody was raised against a peptide within the N-terminal half of human BLIMP1 / PRDM1.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

BLIMP1 / PRDM1 (Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1 Positive regulatory domain I-binding factor 1) is a DNA sequence-specific transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene, as well as other genes. It is required for maturation of B-lymphocytes into immunoglobin secreting plasma cells through direct repression of transcription factors such as Pax5, Bcl6, and Myc, which function in the lineage commitment and proliferation of B cells. BLIMP1 / PRDM1 contains an N-terminal SET domain and four C-terminal C2H2-type zinc-finger motifs. It is also required for effector T-cell function and for the specification of primordial germ cell lineages.

Positive Control

Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for BLIMP1 / PRDM1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

• ChIP = Chromatin Immunoprecipitation;
• ChIP = ChIP Sequencing;
• CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
• CUT&Tag = Cleavage Under Targets and Tagmentation;
• DB = Dot Blot;
• ELISA = Enzyme-linked Immunosorbent Assay;
• EMSA = Electrophoretic Mobility Shift Assay
• FC = Flow Cytometry;
• ICC = Immunocytochemistry;
• IF = Immunofluorescence;
• IHC = Immunohistochemistry;
• IP = Immunoprecipitation;
• MeDIP = Methyl-DNA Immunoprecipitation;
• NEU = Neutralizing;
• WB = Western Blotting