Histone H4R3me2s (symmetric) antibody (pAb)

RRID: AB_2793544
商品コード: 61187 Format: 100 µl ¥92,000 Buy
商品コード: 61188 Format: 10 µl ¥25,000 Buy
商品コード: 61988 Format: 50 µl ¥55,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
10 kDa
Reactivity:
Human, Wide Range Predicted

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Dot Blot Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP, ChIP-Seq
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H4 dimethyl Arg3 (H3R3me2s) antibody was raised against a peptide containing dimethyl Arg3 (symmetric) of human Histone H4.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H4R3me2s (symmetric) antibody (pAb) tested by Western blot.

Histone H4 dimethyl Arg3 symmetric pAb tested by Western blot.
Western blot performed using Histone H4 dimethyl Arg3 symmetric pAb at a dilution of 1:2,000. Nuclear extract from Raji cells (20 µg).

Histone H4R3me2s (symmetric) antibody (pAb) tested by dot blot analysis.

Histone H4 dimethyl Arg3 symmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 symmetric pAb for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 symmetric pAb at 1:3,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified Arg3. Lane 2: monomethyl-Arg3 H4 (symmetric). Lane 3: dimethyl-Arg3 H4 (asymmetric). Lane 4: dimethyl-Arg3 H4 (symmetric)

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4R3me2s (symmetric) antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting