Tet2 antibody (mAb)

RRID: AB_2736901
Clone: 21F11
Aliases: MDS, KIAA1546
Catalog No: 61389 Format: 100 µg ¥72,000 Buy
Catalog No: 61390 Format: 10 µg ¥21,000 Buy
Catalog No: 61990 Format: 50 µg ¥42,000 Buy

Request a quote for a bulk order Request Quote


Antibody Type:
Monoclonal
Isotype:
IgG1, k
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
220 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
IP: 1 - 2 µl per IP
WB: 1 - 2 µg/ml dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ICC/IF
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a recombinant protein corresponding to amino acids 1-300 of human Tet2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

 

Tes2 antibody (mAb) (Clone 21F11) tested by Immunoprecipitation.
Tet2 detection by Immunoprecipitation. Lane 1: Input (25 µg of HL-60 whole cell lysate). Lane 2: Negative control mAb. Lane 3: Tet2 antibody at 1 µg/ml. 250 µg of HL-60 whole cell lysate per IP.

Tes2 antibody (mAb) (Clone 21F11) tested by Western blot.
Tet2 detection by Western blot. Lane 1: HL-60 whole cell lysate (40 µg). Lane 2: Lymphoblastoid Tet2 negative (-/-) whole cell lysate (40 µg). Lane 3: Lymphoblastoid Tet2 positive (+/+) whole cell lysate (40 µg). All probed with Tet2 antibody at a 1 µg/ml dilution.

Background

Tet2 (tet methylcytosine dioxygenase 2) catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation and differentiation in the case of inflammation by regulating gene expression and macrophage differentiation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting