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c-Fos antibody (pAb)
| RRID: AB_2793628 |
| Aliases: p55, AP-1, FOS |
| Catalog No: 61421 | Format: 100 µl | ¥86,000 | Buy |
| Catalog No: 61422 | Format: 10 µl | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:500 - 1:2,000 dilution
Immunogen
This antibody was raised against a peptide within human c-Fos.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
c-Fos (pAb) tested by Western blot.
c-Fos detection by Western blot. The analysis was performed using 20 µg HeLa nuclear extract (TPA stimulated) and c-Fos at a 1:1,000 dilution.
Background
c-Fos (FBJ murine osteosarcoma viral oncogene homolog) is a nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Positive Control
Active Motif's HeLa nuclear extract (TPA stimulated, 2 hr) (Catalog No. 36009) can be used as a positive control for c-Fos antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
