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BRD7 antibody (pAb)
| RRID: AB_3216385 |
| Aliases: Bromodomain containing 7, CELTIX1, BP75, SMARCI1 |
| 商品コード: 65724 | Format: 100 µl | ¥66,800 | Buy |
| 商品コード: 65725 | Format: 10 µl | ¥18,800 | Buy |
| 商品コード: 65924 | Format: 50 µl | ¥39,800 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB*: 1:500 - 1:2,000 dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
*Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This BRD7 antibody was raised against a peptide within the C-terminal region of human BRD7.
Buffer
Purified IgG in 70 mM Tris, 105 mM NaCl, 0.07 mM EDTA, 31 mM glycine, 0.035% sodium azide, 30% glycerol, 0.5mg/mL BSA
BRD7 antibody (pAb) tested by Western blot.
Hela nuclear extract (20 µg) was probed with BRD7 antibody at a 1:2000 dilution.
Background
BRD7 (Bromodomain-containing protein 7, aka CELTIX1) is a chromatin-associated protein component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 inhibit4ed cancer cell growth and metastasis and promotes apoptosis in vitro and in vivo via downregulating AKT pathway. BRD7 may also regulate signaling pathways like ras-raf-MEK-ERK and RB/E2F. Previous studies have also demonstrated that BRD7 was downregulated in human breast cancer, and the downregulation is associated with tumor progression.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
