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Recombinant MAP2K1 (MEK1) protein
| Aliases: Mitogen-Activated Protein Kinase Kinase 1 |
| 商品コード: 81199 | Format: 20 µg | ¥93,100 | Buy |
| 商品コード: 81799 | Format: 1 mg | ¥788,500 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
MAP2K1 (Mitogen-Activated Protein Kinase Kinase 1), also known as MEK1, is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase, and is an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Application Notes
Recombinant MAP2K1 (MEK1) protein is suitable for use in in enzyme kinetics, inhibitor screening, and selectivity profiling.
Protein Details
Recombinant human MAP2K1 (MEK1) protein was expressed in baculovirus expression system as the full length protein (accession number NP_002746.1) with an N-terminal 6×His tag. The molecular weight of the protein is 46.8 kDa.
References
Human
Recombinant MAP2K1 (MEK1) protein
10% SDS-PAGE with Coomassie blue staining
MW: 46.8 kDa
Purity: >80%
HTRF assay for MAP2K1 (MEK1) activity
1 µM TK substrate was incubated with different concentrations of MAP2K1 (MEK1) protein in 10 μl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 μM ATP for 1 hour. Then 10 μl detection reagents containing anti-TK antibody and SAXL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.
HTRF KinEASE TK assay was used to detect the enzymatic activity.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
