Recombinant MGMT protein

Aliases: O-6-Methylguanine-DNA Methyltransferase
Catalog No: 81447 Format: 50 µg ¥88,000 Buy
Catalog No: 81547 Format: 1 mg ¥539,000 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

MGMT(Methylated-DNA--protein-cysteine methyltransferase) Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant MGMT protein that includes full length of human MGMT protein (accession number NP_002403.3) was expressed was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of the protein is 48.48 kDa.

References

Human

 
Recombinant MGMT protein

Recombinant MGMT protein

12.5% SDS-PAGE Coomassie staining

MW: 48.48 kDa

Purity: ≥ 85%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.