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Recombinant HIPK2 (1-640) protein
| Aliases: PRO0593 |
| 商品コード: 81355 | Format: 50 µg | ¥92,000 | Buy |
| 商品コード: 81755 | Format: 1 mg | ¥788,500 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
HIPK2 (Homeodomain Interacting Protein Kinase 2) is a conserved serine/threonine kinase that involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. It acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. HIPK2 phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. It inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation), the phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.
Protein Details
Recombinant HIPK2 (1-640) protein that includes amino acids 1-640 of human HIPK2 protein (accession number NP_001106710.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 69.5 kDa.
Recombinant HIPK2 (1-640) protein
10% SDS-PAGE with Coomassie staining
MW: 69.5 kDa
Purity: >95%
HTRF assay for HIPK2 (1-640) activity
1 µM STK S3 substrate was incubated with different concentrations of HIPK2 (1-640) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. The 10 µl detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
HTRF assay for HIPK2 (1-640) activity
1 µM STK S3 substrate was incubated with different concentrations of HIPK2 (1-640) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. The 10 µl detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
