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Recombinant CDK9 / CyclinT1 protein complex
| Aliases: Cyclin Dependent Kinase 9, TAK, PITALRE, CDC2L4, C-2k, Cell Division Cycle 2-Like Protein Kinase 4, CTK1 |
| 商品コード: 82036 | Format: 20 µg | ¥92,000 | Buy |
| 商品コード: 82636 | Format: 1 mg | ¥788,500 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
CDK9 is a member of the CDC2-like family of kinases. Its cyclin partners are members of the CYCLIN T family (T1, T2a, and T2b) and CYCLIN K. The CDK9/CYCLIN T1 complex is very important in the differentiation programming of several cell types, including muscle cells and neurons, controlling specific differentiation pathways.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant CDK9 / CyclinT1 Complex that includes full length human CDK9 protein (accession number NP_001252.1) without tag and full-length human Cyclin-T1 protein (accession number NP_001231.2) with an N-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK9 and CyclinT1 are 42.76 kDa and 81.95 kDa, respectively.
References
Human
Recombinant CDK9 / CyclinT1 complex
10% SDS-PAGE Coomassie staining
MW: CDK9: 42.76 kDa, Cyclin-T1:81.95 kDa
Purity: ≥ 90%
ADP-Glo assay for CDK9 / CyclinT1 Complex activity
0.2 µg/µl PDKtide peptide substrate was incubated with different concentrations of CDK9 / CyclinT1 Complex protein in a 10 µl reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 µM DTT, 0.1 mg/ml BSA and 100 µM ATP for 1 hour, then add 10 µl ADP-Glo Reagent at RT for 60min.Then add 20 µl Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
