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AbFlex® Histone H3K4me0 antibody (rAb, mouse)
| RRID: AB_2793826 |
| 商品コード: 91267 | Format: 100 µg | ¥72,000 | Buy |
| 商品コード: 91268 | Format: 10 µg | ¥20,000 | Buy |
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Applications
Immunogen
This antibody was raised against a peptide containing the N-terminus of human histone H3.
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
AbFlex® Histone H3K4me0 antibody (rAb) tested by Western blot.
20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me0 antibody at 0.5 µg/ml.
AbFlex® Histone H3K4me0 antibody specificity is shown by Dot Blot.
Recombinant proteins were spotted onto PVDF as follows: Lane 1 - Histone H3 (C110A), Cat. No. 31207; Lane 2 - Histone H3K4me1 (EPL), Cat. No. 31287; Lane 3- Histone H3K4me2 (EPL), Cat. No. 31277; Lane 4 - Histone H3K4me3 (EPL), Cat. No. 31278. Top Panel was probed with AbFlex Histone H3K4me0 antibody at 0.5 µg/ml. Bottom Panel was probed with Histone H3 mAb (Clone 1B1-B2), Cat. No. 61475, at 1 µg/ml.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
