- 受託サービス
- 機能ゲノミクス - LightSwitch™
- 遺伝子発現制御
-
エピジェネティクス・クロマチン
- クロマチン再構築
- クロマチン免疫沈降 (ChIP)
- DNAメチル化解析
-
ヒストン解析
- HATアッセイ(ヒストンアセチルトランスフェラーゼ)
- ヒストン脱アセチル化酵素活性(HDAC)
-
Histone Modification ELISAs
- Total Histone H3 ELISA
- Histone H3 methylated Lys4 ELISA (H3K4)
- Histone H3 acetyl Lys9 ELISA (H3K9)
- Histone H3 methylated Lys9 ELISA (H3K9)
- Histone H3 acetyl Lys14 ELISA (H3K14)
- Histone H3 acetyl Lys27 ELISA (H3K27)
- Histone H3 methylated Lys27 ELISA (H3K27)
- Histone H3 phospho Ser10 ELISA (H3S10ph)
- Histone H3 phospho Ser28 ELISA (H3S28ph)
- Histone Peptide Array
- ヒストン単離
- 組換えヒストンタンパク質
- ヌクレオソーム解析
- 活性化物質 / 阻害物質
- 抗体
- 抗体標識
- リコンビナントタンパク質・酵素
- タンパク質検出・定量
- 細胞・組織・酸抽出物
- 蛍光検出・標識
- サンプル調製
- 形質転換・遺伝子発現
- バイオインフォマティクス
- Fluorescent Dyes and Antibodies
- クリックケミストリー用蛍光ツール
- MAX Stain™ Immunofluorescence Tools
- Super-Resolution Fluorescence Microscopy
- Fluorescent Kits for Cell Biology
-
(871)
Antibodies
- Antibody Type
- Application
- Isotype
- Reactivity
- Modification
- Purification
- Host
- Product Category
- Histone Products
- New Products
- Sample Available
- Antibody Category
-
Research Area
- (464) Chromatin & Histones
- (426) Transcription
- (179) Methylation
- (103) Acetylation & Deacetylation
- (101) Phosphorylation
- (78) Cell Cycle
- (71) DNA Damage & Repair
- (68) Cell Signaling
- (43) Apoptosis
- (21) Nuclear Receptors
- (18) Fluorescence
- (6) Oxidative Stress
- (4) Post Transcription
- (3) Immunoprecipitation
- (1) ELISAs
- (1) SUMOylation
- (1) Ubiquitination
- More
-
(99)
Extracts
- Extract Type
- Extract Species
-
Treatment
- (62) Untreated
- (6) TPA
- (3) IL-6
- (2) Forskolin
- (2) H2O2
- (2) Serum
- (2) TNF-α
- (2) TPA + CI
- (1) Acetaldehyde
- (1) Anacardic acid
- (1) Anisomycin
- (1) CoCl2
- (1) Colcemid
- (1) EGF
- (1) Estradiol
- (1) Etoposide
- (1) Heat shock
- (1) Hypoxia
- (1) IFNγ
- (1) IL-1α
- (1) PHA
- (1) Paclitaxel
- (1) Prolactin
- (1) Retinoic acid
- (1) Sodium butyrate
- (1) Testosterone
- More
-
Cell Line / Tissue
- (18) HeLa
- (7) Hep G2
- (6) Jurkat
- (4) MCF-7
- (4) PC-12
- (3) COS-7
- (3) NIH/3T3
- (3) U-937
- (3) WI-38
- (2) A-431
- (2) C2C12
- (2) F9
- (2) K-562
- (2) LNCaP
- (2) Nb2
- (2) SL-2
- (2) T-47D
- (2) THP-1
- (1) 293
- (1) 3T3-L1
- (1) CCRM-CEM
- (1) Caco-2
- (1) DU 145
- (1) HL-60
- (1) HT-29
- (1) Human Heart
- (1) Human Liver
- (1) Human Lung
- (1) JEG-3
- (1) KG-1
- (1) LoVo
- (1) MDA-MB-231
- (1) MG-63
- (1) MIA PaCa-2
- (1) Mouse Brain
- (1) NIH:OVCAR-3
- (1) P19
- (1) PANC-1
- (1) PC-03
- (1) Raji
- (1) Rat Brain
- (1) Rat Liver
- (1) SK-N-BE
- (1) SW48
- (1) Saos-2
- (1) U-87
- (1) Y-79
- (1) ZR-75-1
- More
- Research Area
- Histone Products
- Modification
- (25) Fluorescent Dyes
-
(326)
Kits
- New Products
-
Product Category
- (94) Chromatin Immunoprecipitation
- (48) Phospho-protein Detection
- (44) Transcription Factor ELISAs
- (42) Sample Preparation
- (25) Epigenetic Services
- (14) DNA Methylation
- (14) Histone ELISAs
- (10) Gelshift / Supershift
- (8) Histone Assays
- (7) Antibody Labeling
- (4) Co-Immunoprecipitation
- (4) Immunofluorescence
- (3) Histone Array
- (2) Chromatin Assembly
- (2) Mitosis Assays
- (1) Nitric Oxide Assays
- (1) Nucleosomes
- (1) Protein Delivery
- (1) Ras GTPase
- (1) miRNA Target Validation
- More
-
Research Area
- (176) Chromatin & Histones
- (114) Transcription
- (102) ELISAs
- (100) Immunoprecipitation
- (93) Accessories
- (78) Sample Preparation
- (77) Phosphorylation
- (60) Cell Signaling
- (48) Methylation
- (23) Cell Cycle
- (20) Apoptosis
- (16) Acetylation & Deacetylation
- (15) Sonication
- (10) DNA Damage & Repair
- (8) Fluorescence
- (3) Nuclear Receptors
- (3) Oxidative Stress
- (2) Gene Regulation
- (1) Clinical Research
- (1) DNA Methylation
- (1) Histone Post Translational Modifications
- (1) Post Transcription
- More
- Histone Products
- Modification
- Product Class
-
(599)
Recombinant Proteins
- New Products
-
Protein Category
- (201) Histone
- (158) Transcription
- (57) Bromodomain
- (55) Histone Methyltransferase (HMT)
- (25) Kinase
- (24) Histone Deacetylase (HDAC)
- (20) Histone Demethylase (HDM)
- (18) DNA Damage
- (16) Nuclear Receptor
- (10) Histone Acetyltransferase (HAT)
- (9) DNA Methyltransferase (DNMT)
- (5) Cytosine Oxygenase (TET)
- (1) Glucosyltransferase
- (1) Neddylation
- (1) Ubiquitination
- More
- Expressed In
- Protein Species
- Modification
-
Research Area
- (385) Chromatin & Histones
- (239) Methylation
- (201) Transcription
- (127) Acetylation & Deacetylation
- (43) Phosphorylation
- (39) Cell Signaling
- (25) Post Transcription
- (21) Cell Cycle
- (16) Nuclear Receptors
- (14) DNA Damage & Repair
- (12) Oxidative Stress
- (9) Apoptosis
- (3) Ubiquitination
- (2) Sample Preparation
- More
- Histone Products
-
(149)
Reporter Assays
- LightSwitch™ Collections
-
Product Category
- (92) Synthetic Response Elements
- (11) Pathway Biomarker Sets
- (8) LightSwitch™ 3´UTR Controls
- (8) LightSwitch™ Promoter Controls
- (8) LightSwitch™ Reporter Collections
- (8) Stable Reporter Cell Lines
- (4) LightSwitch™ Empty Vectors
- (3) Gene Regulation Services
- (3) Transfection Reagents
- (2) miRNA Mimics and Inhibitors
- (1) LightSwitch™ Assay Kits
- (1) Synthetic miRNA Targets
- More
- Research Area
- miRNA Products
- Type of Control
- (59) Small Molecules
リコンビナントタンパク質・酵素

- リコンビナントタンパク質・酵素
- »
- Recombinant Proteins
- »
- Recombinant Histone H3K4me3 (EPL)
Request a quote for a bulk order
Expressed Protein Ligation
Recombinant Histone H3K4me3 (EPL) has been generated using the patented Expressed Protein Ligation (EPL) technology. EPL can be used to generate methylated, acetylated or phosphorylated histones. In EPL, the histone globular domain is ligated to a peptide that contains the N-terminal histone tail with the desired site-specific modification. The ligation reaction maintains the native histone bonds, and the resulting recombinant protein more closely mimics natural histones.
Contents
25 µg supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
Application Notes
Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
Protein Details
Recombinant Histone H3 trimethyl Lys4 (H3K4me3) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a N-terminal histone tail peptide containing trimethyl lysine 4 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,267 Daltons. Please click on the image below to view the mass spectrum.
References
This product was used in the following publications:
Nucleic Acids Res. (2019). Jun 5. pii: gkz493. PMID: 31165872. (Histone Demethylation Assay)
Nature (2019). 567(7749):535-539. PMID: 30867594. (Histone Methyltransferase Assay (HMT)
Recombinant Histone H3 trimethyl Lys4 analyzed by SDS-PAGE gel.
SDS-PAGE analysis of 2 µg Recombinant Histone H3 trimethyl Lys4 (lane 1) and 1 µg Recombinant Histone H3 trimethyl Lys4 (lane 2).
Storage
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
RELATED PRODUCTS
Technical Data Sheet
Recombinant Histone H3K4me3 (EPL)Data Thumbnails
SDS-PAGE gel of Recombinant Histone H3 trimethyl Lys4.
(Click image to enlarge and see details.)