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Histone H3K4me3 antibody (mAb)
| RRID: AB_2793611 |
| Clone: MABI 0304 |
| 商品コード: 61379 | Format: 100 µg | ¥92,000 | Buy |
| 商品コード: 61979 | Format: 50 µg | ¥55,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
CUT&Tag
CUT&RUN
ChIP-qPCR
ICC/IF
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 trimethyl Lys4 antibody was raised against a synthetic peptide containing trimethyl Lys4 of human histone H3.
Buffer
PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Histone H3K4me3 antibody (mAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 ug of chromatin from a human medulloblastoma cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify Histone H3K4me3 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.
Histone H3 trimethyl Lys4 antibody tested by Western blot. HeLa acid extract (20 µg) probed with Histone H3K4me3 antibody (10 µg/ml dilution).
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me3 antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
This antibody is manufactured by MAB Institute, Inc.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
