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Recombinant HDAC5 protein
| Aliases: HDAC-5, Histone Deacetylase 5, HD5 |
| 商品コード: 31534 | Format: 10 µg | ¥96,000 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Protein Details
Recombinant human HDAC5 was expressed in a baculovirus expression system as the full length protein (accession number NP_001015053.1) with an N-terminal FLAG-Tag. The molecular weight of the protein is 124 kDa. The purity of HDAC5 protein is >66% by SDS-PAGE.
Recombinant HDAC5 protein gel.
HDAC5 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.
MW: 124 kDa
Purity: >70%
HDAC-Glo™ Class IIa Assay for HDAC5 activity
Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 μM substrate was incubated with different concentrations of HDAC5 protein and 1/20000 developer reagent in 20 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 20 min. All the operations and reactions were performed at room temperature. HDAC-Glo™ Class IIa assay was used for detection.
HDAC-Glo™ Class IIa Assay for HDAC5 activity
Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 μM substrate was incubated with different concentrations of HDAC5 protein and 1/20000 developer reagent in 20 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 20 min. All the operations and reactions were performed at room temperature. HDAC-Glo™ Class IIa assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
