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Histone H3ac (pan-acetyl) antibody (pAb)
| RRID: AB_2687871 |
| 商品コード: 39139 | Format: 100 µl | ¥92,000 | Buy |
| 商品コード: 39140 | Format: 10 µl | ¥25,000 | Buy |
| 商品コード: 39040 | Format: 50 µl | ¥55,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-qPCR, ChIP-Seq
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 acetyl antibody was raised against a peptide including acetyl-lysines contained in the N-terminal tail of histone H3.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 61637) of this antibody that was purified by Protein A Chromatography is also available.
Histone H3ac (pan-acetyl) antibody (pAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 µg of Human iPSC cell chromatin and 5 µl of Histone H3ac (pan-acetyl) antibody. ChIP DNA was sequenced on the Illumina NextSeq and 23 million sequence tags were mapped to identify Histone H3ac (pan-acetyl) binding sites on chromosome 12.
Histone H3ac (pan-acetyl) antibody (pAb) tested by Western blot.
Detection of acetylated Histone H3 by Western blot analysis using 20 µg of Hela nuclear extracts and Histone H3 acetyl antibody at a 1:5,000 dilution.
Lane 1: Untreated HeLa cells.
Lane 2: HeLa cells treated with sodium butyrate.
Histone H3ac (pan-acetyl) antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3ac antibody. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto Nitrocellulose and probed with the antibody at a dilution of 1:3,000. The amount of peptide (picomoles) spotted is indicated (picomoles).
. Lane 1: H3K4ac. Lane 2: Unmodified H3. Lane 3: H3K9ac. Lane 4: Unmodified H3. Lane 5: H3K14ac. Lane 6: Unmodified H3K14. Lane 7: H3K18ac. Lane 8: Unmodified H3K18ac. Lane 9: H3K23ac. Lane 10: Unmodified H3K23ac. Lane 11: H3K27ac. Lane 12: Unmodified H3K27ac.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3ac (pan-acetyl) antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
