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Histone H3ac (pan-acetyl) antibody (pAb)
| RRID: AB_2793714 |
| 商品コード: 61637 | Format: 100 µg | ¥92,000 | Buy |
| 商品コード: 61638 | Format: 10 µg | ¥25,000 | Buy |
| 商品コード: 61937 | Format: 50 µg | ¥55,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-qPCR
IHC
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This antibody was raised against a peptide including acetyl-lysines contained in the N-terminal tail of human Histone H3.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a sera version (Catalog No. 39139) of this antibody is also available.
H3ac (pan-acetyl) antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 13 µg of chromatin from both HeLa cells and 10 µg H3ac (pan-acetyl) antibody. ChIP DNA was used in qPCR with the gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
Histone H3ac (pan-acetyl) antibody (pAb) tested by Western blot.
Detection of acetylated Histone H3 by Western blot analysis using 20 µg HeLa nuclear extract and Histone H3ac (pan-acetyl) antibody at a 1 µg/ml dilution.
Lane 1: Nuclear extract of untreated HeLa cells.
Lane 2: Nuclear extract of HeLa cells treated with sodium butyrate.
Histone H3ac (pan-acetyl)antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3ac antibody. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to the row.
Lane 1: H3K4ac peptide. Lane 2: unmodified H3K4 peptide. Lane 3: H3K9ac peptide. Lane 4: unmodified H3K9 peptide. Lane 5: H3K18ac peptide. Lane 6: unmodified H3K18 peptide. Lane 7: H3K23ac peptide. Lane 8: unmodified H3K23 peptide. Lane 9: H3K27ac peptide. Lane 10: unmodified H3K27 peptide.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3ac (pan-acetyl) antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
