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Histone H3K27me3 antibody (pAb)
| RRID: AB_2561020 |
| 商品コード: 39155 | Format: 100 µg | ¥92,000 | Buy |
| 商品コード: 39055 | Format: 50 µg | ¥55,000 | Buy |
| 商品コード: 39157 | Format: 10 µg | ¥25,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
CUT&Tag
ChIP, ChIP-Seq
Native ChIP, CUT&RUN
WB
ICC/IF
IHC(P), IHC(F)
ELISA
RNA FISH
Proximity Ligation Assay (PLA)
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3. testing
Buffer
Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39156) of this antibody is also available.
Histone H3K27me3 pAb tested by ChIP-Seq.
ChIP was performed using chromatin from the human iPS 19.11 cell line. ChIP DNA was sequenced on the Illumina GA II and sequence tags were mapped to identify H3K27me3 binding. The image shows H3K27me3 binding across a 140,000 bp region of the HOX gene cluster on chromosome 12.
Histone H3K27me3 pAb tested by immunofluorescence.
HeLa cells stained with Histone H3 trimethyl Lys27 antibody (1.7 µg/ml dilution).
Top panel: Histone H3 trimethyl Lys27 antibody.
Middle: DAPI.
Bottom: Merge of both images.
Histone H3K27me3 pAb tested by Western blot.
HeLa cell nuclear extract (20 µg) probed with Histone H3K27me3 pAb at 0.5 µg/ml.
Histone H3 trimethyl Lys27 antibody specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 trimethyl Lys27 antibody was applied at a dilution of 1:5,000 to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the H3 trimethyl Lys27 peptides by the antibody blocked by phosphorylation at Ser28.
Array Data File
Histone H3K27me3 antibody tested by Sysmex Corporation using their Super Resolution Microscope HM-1000
HeLa cells were stained with Histone H3 trimethyl Lys27 pAb, with Janelia Fluor 646 (orange) conjugated anti-rabbit IgG as a secondary antibody. Images were obtained at an exposure time of 30 milliseconds.
qPCR Primers
Active Motif's Human Positive Control Primer Set MYT1 (Cat. No. 71007) and Human Negative Control Primer Set 3 (Cat. No. 71023) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Pax-2 (Cat. No. 71020) , Mouse Negative Control Primer Set 3 (Cat. No. 71013) and Mouse Negative Control Primer Set 4 (Cat. No. 71014) have been validated for both qPCR and endpoint PCR.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K27me3 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
