Histone H3K9me3 antibody (mAb)

RRID: AB_2935892
Clone: 2AG-6F12-H4
商品コード: 39285 Format: 100 µl ¥66,800 Buy
商品コード: 39286 Format: 10 µl ¥18,800 Buy

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Monoclonal
Isotype:
IgG1, k
Purification:
Culture Supernatant
Host:
Mouse
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IF
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H3 trimethyl Lys9 antibody was raised against a peptide containing trimethyl-lysine 9 of human histone H3.

Buffer

Culture supernatant containing 30% glycerol, 0.035% sodium azide.

View our Guide to Histone Modifications and Biological Function.

 
Histone H3K9me3 antibody (mAb) tested by immunofluorescence.

Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) tested by immunofluorescence.
Staining of HeLa cells with Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) (1:250 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

Histone H3K9me3 antibody (mAb) tested by Western blot.

Histone H3K9me3 (Clone 2AG-6F12-H4) tested by Western blot.
HeLa cell acid extract (20 µg) probed with Histone H3K9me3 antibody at 1:1000 dilution.

Histone H3K9me3 antibody (mAb) tested by dot blot analysis.

Histone H3K9me3 (Clone 2AG-6F12-H4) tested by dot blot analysis.
Various methylated H3 proteins were spotted onto PVDF and probed with antibody at 1:5000 dilution. The amount of protein (picomoles) spotted is indicated next to each row.
Column 1: Histone H3 (Cat. No. 31294) .
Column 2: Histone H3K9me1 (Cat. No. 31281).
Column 3: Histone H3K9me2 (Cat. No. 31280).
Column 4: Histone H3K9me3 (Cat. No. 31586).
Column 5: Histone H3K4me3 (Cat. No. 31278).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting