Histone H3K14bz antibody (pAb)

RRID: AB_3216391
商品コード: 39303 Format: 100 µl ¥66,800 Buy
商品コード: 39304 Format: 10 µl ¥18,800 Buy
商品コード: 39333 Format: 50 µl ¥39,800 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Wide Range Predicted

Applications

Western Blot Validated Dot Blot Validated

Immunogen

This antibody was raised against a peptide including benzyol- lysine 14 of human histone H3.

Buffer

70 mM Tris, 105 mM NaCl, 0.07 mM EDTA, 31 mM glycine, 0.035% sodium azide, 0.35 mg/mL BSA, and 30% glycerol.

 
Histone H3K14bz antibody (pAb) tested by Western blot.

Histone H3K14bz antibody (pAb) tested by Western blot.
20 µg of HeLa nuclear extract was run on SDS-PAGE and probed with antibody at 1:500 dilution. Lane 1: untreated cells. Lane 2: cells treated with Sodium Benzoate.

Histone H3K14bz antibody (pAb) tested by dot blot analysis.

Specificity Analysis of Histone H3K14bz antibody (pAb):
Dot blot analysis was used to confirm the specificity of the antibody (pAb) for benzoylated (bz) lysine14 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto nitrocellulose and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Columns spotted as follows: 1: H3K23 unmod. 2: H3K23bz. 3: H4K8 unmod 4: H3K8bz. 5: H3K12 unmod. 6: H3K12bz. 7: H3K14 unmod. 8: H3K14bz. 9: H2AK9 unmod. 10: H2AK9bz.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting