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Histone H3K4ac antibody (pAb)
| RRID: AB_2793236 |
| 商品コード: 39381 | Format: 100 µl | ¥69,000 | Buy |
| 商品コード: 39382 | Format: 10 µl | ¥18,800 | Buy |
| 商品コード: 39481 | Format: 50 µl | ¥41,300 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP, ChIP-Seq
CUT&Tag
IF
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 acetyl Lys4 antibody was raised against a peptide containing acetyl-lysine 4 of histone H3.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Histone H3K4ac antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from a human medulloblastoma cell line and 4 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Histone H3K4ac binding sites. The image shows binding of GAPDH on chromosome 12.
Histone H3K4ac antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H3 acetyl Lys4 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H3K4ac antibody (pAb) tested by immunofluorescence.
Top left: HeLa cells stained with Histone H3 acetyl Lys4 pAb (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.
Histone H3K4ac antibody (pAb) tested by Western blot.
Western Blot: A549 whole-cell extract (20 µg per lane) probed with Histone H3 acetyl Lys4 pAb (1:2,000 dilution).
Lane 1: Untreated cells.
Lane 2: Cells treated with Trichostatin A.
Histone H3K4ac antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys4 pAb for acetyl Lys4 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl lysine 4 peptide. Lane 2: unmodified lysine 4 peptide. Lane 3: acetyl lysine 9,14,18 peptide. Lane 4: unmodified lysine 9,14,18 peptide. Lane 5: acetyl lysine 9 peptide. Lane 6: acetyl lysine 14 peptide. Lane 7: acetyl lysine 18 peptide. Lane 8: acetyl lysine 23 peptide. Lane 9: acetyl lysine 27 peptide.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4ac antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
