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Histone H3K9me1 antibody (pAb)
| RRID: AB_2793381 |
| 商品コード: 39887 | Format: 100 µg | ¥69,000 | Buy |
| 商品コード: 39888 | Format: 10 µg | ¥18,800 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 monomethyl Lys9 antibody was raised against a peptide including monomethyl lysine 9 of histone H3.
Buffer
Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39249) of this antibody is also available.
View our Guide to Histone Modifications and Biological Function.
Histone H3K9me1 antibody (pAb) tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human breast cancer cell line (4.5 million cells) and 4 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The literature suggests that this histone modification is enriched in actively transcribed genes. The 2 images above show enrichment of H3K9me1 across gene bodies.
Histone H3 monomethyl Lys9 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) probed with Histone H3 monomethyl Lys9 antibody at a dilution of 2 µg/ml.
Histone H3 monomethyl Lys9 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys9 antibody for monomethyl Lys9 of histone H3. Recombinant methylated proteins corresponding to the immunogen and related proteins were spotted onto PVDF and probed with Histone H3 monomethyl Lys9 antibody at 2 µg/ml. The amount of protein (picomoles) spotted is indicated next to each row.
Lane 1: recombinant monomethyl lysine 9 protein.
Lane 2: recombinant dimethyl lysine 9 protein.
Lane 3: recombinant trimethyl lysine 9 protein.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9me1 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
