Menin antibody (pAb)

RRID: AB_2615023
Aliases: MEN1, MEAI, SCG2
商品コード: 61005 Format: 100 µl ¥69,000 Buy
商品コード: 61006 Format: 10 µl ¥18,800 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
80 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 5 µl per ChIP
ChIP-Seq & ChIP-chip: 5 µl each
WB*: 1:500 – 1:1,000 dilution

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Immunogen

This Menin antibody was raised against a peptide corresponding to amino acids within the C-terminal region of human Menin.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
Menin antibody (pAb) tested by ChIP-Seq.

Menin antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 µg of chromatin from a mouse thymus and 5 µg of MEN1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify MEN1 binding sites. MEN1 is a subunit of the MLL complex which catalyzes H3K4 trimethylation and is therefore expected to localize with H3K4me3. A sampling of the MEN1 ChIP-Seq data shows the expected co-localization of MEN1 and H3K4me3.

Menin antibody (pAb) tested by Western blot.

Western blot of Menin antibody.
Nuclear extract of HEK293 cells (20 μg) probed with Menin antibody (1:1,000).

Background

Menin (MEN1, SCG2) is a tumor suppressor and a component of multiple MLL-containing protein complexes (MLL, ACSOM, MLL2/3 and MLL3/4), which methylate histone H3 at lysine 4. Menin interacts directly with MLL through a domain in the N-terminus of MLL. It also interacts with RNA polymerase II large subunit C-terminal domain phosphorylated at Ser5. Menin is involved in the activation of HOX gene expression and is localized along with MLL at several HOX gene promoters. It is also important for activating the expression of several cell cycle regulators, indicating a mechanism for its role as a tumor suppressor. Defects in Menin are associated with inherited Multiple Endocrine Neoplasia type I, a dominant predisposition to cancer of a number of endocrine tissues.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Menin antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Menin antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting