5-Formylcytosine (5-fC) antibody (pAb)

RRID: AB_2793558
Catalog No: 61227 Format: 100 µg ¥70,000 Buy
Catalog No: 61228 Format: 10 µg ¥20,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Reactivity:
Not Species Specific

Applications

Western Blot Validated Dot Blot Validated

Application Notes


Validated Applications:
DB: 0.5 - 2 µg/ml dilution

Published Applications:
WB
See references for more information. Individual optimization may be required.

Immunogen

This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

Dot blot of 5-Formylcytosine pAb.
Dot blot analysis was used to confirm the specificity of 5-Formylcytosine antibody for 5-Formylcytosine (5-formylcytidine, 5-fC). Single-stranded 38 nt DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) corresponding to the immunogen and related sequences were spotted onto nitrocellulose and probed with the antibody at 0.2 µg/ml. Lane 1: oligo containing 5-carboxylcytidine. Lane 2: oligo containing 5-formylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane 4: oligo containing 5-methylcytidine. Lane 5: oligo containing unmodified cytidine.

Background

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.

A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting