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SETD7 / SET7 antibody (pAb)

Aliases: SET Domain Containing Lysine Methyltransferase 7, KMT7, SET9, SET7/9
商品コード: 61451 Format: 100 µl ¥68,400 Buy Now
商品コード: 61452 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:60 kDa


Western Blot Validated

for SETD7 / SET7 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for SETD7 / SET7 antibody (pAb)Immunogen

This antibody was raised against a peptide within human SETD7 / SET7.

for SETD7 / SET7 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SETD7 / SET7 antibody (pAb)References

SETD7 / SET7 antibody (pAb) tested by Western blot.

SETD7 / SET7 (pAb) tested by Western blot.
Detection of SETD7 / SET7 by Western blot analysis. Nuclear extract of MCF-7 cells (20 µg) was probed with SETD7 / SET7 (pAb) at a 1:500 dilution.

for SETD7 / SET7 antibody (pAb)Background

SETD7 / SET7 (SET Domain Containing (Lysine Methyltransferase) 7) is a histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.

for SETD7 / SET7 antibody (pAb)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for SETD7 / SET7 antibody.

for SETD7 / SET7 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SETD7 / SET7 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

SETD7 / SET7 antibody (pAb)

Data Thumbnails

SETD7 / SET7 antibody (pAb) tested by Western blot.

Western blot of SETD7 / SET7 pAb.
(Click image to enlarge and see details.)