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Histone H3K4me1 antibody (pAb)
| RRID: AB_3216367 |
| 商品コード: 61781 | Format: 100 µg | ¥92,000 | Buy |
| 商品コード: 61782 | Format: 10 µg | ¥25,000 | Buy |
| 商品コード: 61811 | Format: 50 µg | ¥55,000 | Buy |
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Applications
Immunogen
This antibody was raised against a peptide containing monomethyl-lysine 4 of Histone H3.
Buffer
Purified IgG in PBS containing 30% glycerol and 0.035% sodium azide.
Histone H3K4me1 antibody (pAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 µg of HeLa cell chromatin and 5 µg of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.1 million sequence tags were mapped to identify Histone H3K4me1 binding sites on chromosome 1. Histone H3K4me1 antibody Cat. No. 61781 is a Protein A purified format generated from our serum format of same antibody, Cat. No. 39299.
Histone H3K4me1 antibody (pAb) tested by Luminex bead-based specificity analysis.
Various recombinant Histone proteins were conjugated to Luminex beads and incubated with varying dilutions of Histone H3K4me1 antibody. Bound antibody was detected with anti-rabbit IgG-PE and read in a MagPix Luminex Instrument.
Luminex® is a registered trademark of Luminex Corporation.
Histone H3K4me1 antibody (pAb) tested by Western blot.
20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with Histone H3K4me1 antibody at 0.5 µg/ml.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me1 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
