Histone H3.3K27M antibody (pAb)

RRID: AB_2793773
商品コード: 61803 Format: 100 µl ¥66,800 Buy
商品コード: 61804 Format: 10 µl ¥18,800 Buy

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Reactivity:
Human

Applications

Western Blot Validated

Immunogen

This antibody was generated using a peptide containing a methionine substitution at lysine 27 of human Histone H3.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H3.3K27M antibody (pAb) tested by Western blot.

Western blot analysis of Hantibody specificity for Histone H3.3K27M
0.1 µg per lane of Recombinant Human Histone H3.3 Cat. No. 31295 (Lanes 1 & 3) or Recombinant Human Histone H3.3K27M Cat. No. 31552 (Lanes 2 & 4) were probed with AbFlex® Histone H3.3 antibody (rAb), Cat. No. 91191 at 2µg/ml (Lanes 1 & 2) or Histone H3.3K27M antibody (pAb) at 1:20,000 dilution  

Histone H3.3K27M antibody (pAb) tested by Western blot.

Western blot of Histone H3.3K27M pAb.
H1299 cell extracts transfected with human Histone H3.3 wild-type (wt) or various Histone H3.3 single amino acid substitutions were probed with Histone H3.3K27M antibody (pAb) at 1:500. Beta-actin was used as a loading control.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting