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Recombinant MBD4-MBD protein
| Aliases: Methyl-CpG-binding domain protein 4, MED1 |
| Catalog No: 81473 | Format: 100 µg | ¥89,000 | Buy |
| Catalog No: 81573 | Format: 1 mg | ¥539,000 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
MBD4 (Methyl-CpG-binding domain protein 4) can mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methylCpG-binding protein.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant MBD4-MBD protein that includes amino acids 71-159 of human MBD4 protein (accession number NP_003916.1) was expressed in E. coli cells with an N-terminal 6xHis tag. The molecular weight of the protein is 12.31 kDa.
References
Human
MBD4-MBD protein gel.
12.5% SDS-PAGE gel stained with Coomassie Blue.
MW: 12.31 kDa
Purity: >95%
HTRF assay for MBD4-MBD activity
1 μM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD4-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10 μl detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
