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Recombinant STUB1 / CHIP protein
| Aliases: CHIP, SDCCAG7, UBOX1, E3 Ubiquitin Protein Ligase CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7 |
| 商品コード: 82008 | Format: 100 µg | ¥92,000 | Buy |
| 商品コード: 82608 | Format: 1 mg | ¥560,500 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
STUB1 (E3 ubiquitin-protein ligase CHIP) also known as CHIP, HSPABP2, NY-CO-7, SCA48, SCAR16, SDCCAG7 and UBOX1. STUB1 is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates ICER-type isoforms of CREM and targets them for proteasomal degradation, thereby acts as a positive effector of MAPK/ERK-mediated inhibition of apoptosis in cardiomyocytes. Inhibits lipopolysaccharide-induced apoptosis and hypertrophy in cardiomyocytes, via ubiquitination and subsequent proteasomal degradation of NFATC3. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant STUB1 / CHIP protein that includes full length of human STUB1 protein (accession number NP_005852.2) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 37.02 kDa. The purity of the protein is ≥ 95% by SDS-PAGE.
References
Human
Recombinant STUB1 / CHIP protein
12.5% SDS-PAGE Coomassie staining
MW: 37.02 kDa,
Purity: ≥ 95%
ELISA for STUB1 / CHIP activity
ELISA plate was coated with 50 ng HSC70 protein in each well. Then 63 nM UBA1, 1 μM UBE2D1, 7.9 μM Ubiquitin and 25 μM ATP and different concentrations of STUB1 protein in 50 μl reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA were added in each well and incubated at 37℃ for 1 hour. Anti-Ubiquitin (Cat.39741) antibody was used to detect reaction products.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
