Recombinant EEF1AKMT4 (2-255) protein

Aliases: EEF1A lysine methyltransferase 4, Eukaryotic Translation Elongation Factor 1 Alpha Lysine Specific Methyltransferase 4
商品コード: 82014 Format: 100 µg ¥93,100 Buy
商品コード: 82614 Format: 1 mg ¥608,000 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

eEF1AKMT4 (EEF1A lysine methyltransferase 4) is a Protein-lysine methyltransferase that efficiently catalyzes three successive methylations on 'Lys-36' in eukaryotic translation elongation factor 1 alpha (EEF1A1 or EEF1A2).

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant eEF1AKMT4 (2-255) protein that includes amino acids 2-255 of human eEF1AKMT4 protein (accession number NP_115707.2) was expressed in E. coli and contains an N-terminal His and FLAG tag with a molecular weight of 31.46 kDa. The purity of the protein is ≥ 90% by SDS-PAGE.

References

Human

 
Recombinant eEF1AKMT4 protein Coomassie gel

Recombinant eEF1AKMT4 (2-255) protein 12.5% SDS-PAGE Coomassie staining

MW: 31.46 kDa

Purity: ≥ 90%

Activity assay for eEF1AKMT4 protein activity

Recombinant eEF1AKMT4 (2-255) protein activity assay

2 µM Recombinant eEF1A1 (Cat. No. 81219) was incubated with 0 µM, 0.5 µM, 1 µM, and 2 µM eEF1AKMT4 (2-255) protein in a 30 µl reaction system containing 50 mM Tris-HCl pH 7.8, 50 mM KCl, 5 mM MgCl2, 1 mM GTP and 50 µM SAM for 1 hr at 37℃ and 3 hr at RT. Reaction products were detected by Western blot using eEF1A K36me3 antibody (in-house).

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.