Recombinant SUMO3 protein - biotinylated

Aliases: SMT3A, SMT3H1, Small ubiquitin related modifiers, Smt3B
商品コード: 82107 Format: 100 µg ¥92,000 Buy
商品コード: 82707 Format: 1 mg ¥560,500 Buy

Request a quote for a bulk order Request Quote

Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

SUMO3 (Small ubiquitin-related modifier 3) is a ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Plays a role in the regulation of sumoylation status of SETX.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

Protein Details

Recombinant SUMO3 protein - biotinylated that includes full length of human SUMO3 protein (accession number NP_008867.2) was expressed in E. coli and contains an N-terminal His & Avi tag with a molecular weight of 15.89 kDa. This protein has been modified by biotin in vivo. The purity of the protein is ≥ 90% by SDS-PAGE.

References

Human

 
Recombinant SUMO3 protein gel.

Recombinant SUMO3 protein gel.
SUMO3 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.
MW: 15.89 kDa
Purity: >90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.