Recombinant SUMO2 protein - biotinylated

Aliases: Small ubiquitin-related modifier 2, SMT3B, SMT3 Homolog 2
商品コード: 82106 Format: 100 µg ¥92,000 Buy
商品コード: 82706 Format: 1 mg ¥560,500 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

SUMO2 (Small ubiquitin-related modifier 2) is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post translational modification on lysine residues of proteins plays a crucial role in several cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Plays a role in the regulation of sumoylation status of SETX.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

Protein Details

Recombinant SUMO2 protein - biotinylated that includes full length of human SUMO2 protein (accession number NP_008868.3) was expressed in E. coli and contains an N-terminal His & Avi tag with a molecular weight of 15.12 kDa. This protein has been modified by biotin in vivo. The purity of the protein is ≥ 70% by SDS-PAGE.

References

Human

 
Recombinant SUMO2 protein gel.

Recombinant SUMO2 protein gel.
SUMO2 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.
MW: 15.12 kDa
Purity: >70%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.