AbFlex® Histone H4K20me1 antibody (rAb)

RRID: AB_3216320
商品コード: 91291 Format: 100 µg ¥72,000 Buy
商品コード: 91292 Format: 10 µg ¥20,000 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
12 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated

Immunogen

This antibody was raised against a peptide containing monomethyl-lysine 20 of human Histone H4.

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.

 
AbFlex<sup>®</sup> Histone H4K20me1 antibody tested by Western blot.

AbFlex® Histone H4K20me1 antibody tested by Western blot.
20 µg of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H4K20me1 antibody at 0.5 µg/ml.

AbFlex<sup>®</sup> Histone H4K20me1 antibody specificity tested by peptide array analysis

AbFlex® Histone H4K20me1 antibody specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me1 antibody was applied at 0.1 µg/ml to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting