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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.5 - 2 µg/ml
ICC: 2 - 10 µg/ml
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
Acetyllysine-BSA
Buffer
PBS containing 50% Glycerol, 1% BSA, and 0.09% sodium azide.
Western blot of Acetyl-Lysine antibody (rAb).
HeLa cells nontreated or treated with Trichostatin A (TSA), were probed using Acetyl-Lysine antibody (RM101) at 0.5 µg/ml.
Immunofluorescence stain of Acetyl-Lysine antibody (rAb).
HeLa cells were stained using Acetyl-Lysine antibody (RM101) at 2 µg/ml (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).
Background
Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histones can occur at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as HAT1 or GCN5. Acetylation of histones is often associated with transcriptional activation.
Clone RM101 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysines with other modifications.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
