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AbFlex® PARP1 antibody (rAb)
| Aliases: PARP, PPOL, ADPRT, ARTD1, ADPRT1, ADPRT 1, pADPRT-1 |
| 商品コード: 91479 | Format: 100 µg | ¥72,000 | Buy |
| 商品コード: 91480 | Format: 10 µg | ¥20,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.5 - 2 µg/ml
IP: 2 µg per IP
IF:2 µg/ml
Immunogen
This PARP1 antibody was raised against a peptide of human PARP1.
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
AbFlex® PARP1 antibody (rAb) tested by Western Blot.
Detection of PARP1 by Western blot analysis using 40 µg of HEK293 (Lane 1) and Raji (Lane 2) whole cell extracts and probed with antibody at 0.5 µg/ml.
AbFlex® PARP1 rAb antibody tested by immunoprecipitation.
2 µg of antibody was used to immunoprecipitate PARP1 from 1 mg of HEK293F whole cell extract, 40% IP product (Lane 2) was run on SDS-PAGE and probed with the same antibody at 0.5 µg /ml. Tween-20 at 0.1% was added to blocking buffers to perform Western blot and reduce background. 2 µg of rabbit IgG was used as negative control for IP (Lane 1).
AbFlex® PARP1 rAb antibody tested by immunofluorescence.
PARP1 was detected in human HeLa cells using AbFlex PARP1 (rAb) at 2 µg/mL, followed by Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L).
Background
AbFlex®antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
AbFlex® PARP1 antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells.
PARP1 (Poly (ADP-Ribose) Polymerase 1, also known as PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1) is a chromatin-associated enzyme, poly(ADP-ribosyl) transferase, which modifies various nuclear proteins by poly(ADP-ribosyl) ation. Poly (ADP-ribosyl) ation is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and in the regulation of the molecular events involved in the recovery of cells from DNA damage.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
